RE: Disposable microtome blades for GMA sectioning
I have never been successful using disposable blades for GMA and I have
tried many. I don't think the holding systems are strong enough for GMA.
From: Gayle Callis [mailto:email@example.com]
Sent: Friday, April 11, 2003 3:04 PM
Subject: Disposable microtome blades for GMA sectioning
Have a lady who needs to do GMA embedded embryos, but her microtome is
probably going to be the older model Leitz 1512. I know the microtome was
a workhorse for some labs when sectioning GMA embedded tissues. The only
setup I observed with 1512 was using triangular glass knives. However, I
am not sure she has a triangular knife holder for glass knives. Tungsten
carbide d profile or even c profile is an option.
If you did this, were you successful when using disposable microtome
blades, low or high profile?
Research Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
S. 19th and Lincoln St
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
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