Hi Gayle,

You're absolutely right about the NovaRed not being AEC.  I was merely looking at the possibility of an HRP chromogen that is red.  The researchers don't give a hoot about what we call it, so long as they get the color reaction they want.

If Dr. van der Loos' protocol is the same one as in his Immunoenzyme Multiple Staining book, I have it and think I will give it a try.  How stable is it stored at 4 degrees C (months?)?

Teri

-----Original Message-----
From: Gayle Callis [mailto:gcallis@montana.edu]
Sent: Wednesday, April 30, 2003 9:43 AM
To: histonet@pathology.swmed.edu
Subject: AEC versus NovaRed


Teri 

First, and sorry for the correction, but Vector NovaRed is not AEC, it is a
proprietary mixture for use with HRP methods and gives a brick red color
rather than the clear red-orange of AEC. 

As for price increase on AEC+, we changed over to in house preparation of
AEC using Dr. Chris van der Loos's protocol but not because of the price.
It is as sensitive or more so than AEC+.  I realize one is dealing with
mixing hazardous chemicals, but our results warrant using it with care
taken while making it up.  I have found AEC+ to go bad i.e. contamination
without extreme care in handling, but I loved it for ready to use
convenience and sensitivity.  Contamination is probably due to not using it
up quickly as compared to rapid use in bigger laboratory.  I must admit
AEC+ is great for those who can afford or like results from using it.  

We now develop AEC in a coplin jar with the positive control being observed
with microscope.  Coplin jar lets any ppt fall to bottom of jar. I am sure
either Chris or myself (with his permission) will attach you the protocol. 

 
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
S. 19th and Lincoln St
Bozeman MT 59717-3610

406 994-6367 (lab with voice mail)
406 994-4303 (FAX)

email: gcallis@montana.edu