In vivo proliferation in vascular sections using Amersham BrdU ki t

From:"Shaposhnik, Zory"


I have tried to use the Amersham BrdU kit to look at proliferation in frozen
mouse aortic sections and have not seen any staining with DAB. 5uM spleen
sections were used as a positive control. No staining was seen there either.
I confirmed that the Peroxidase linked secondary anti-mouse Ig 2a antibody

Does anyone have any suggestions? Is a PCNA stain an easier way to look at
proliferating cells in frozen sections? If so, what kit/technique would be

Thank you,

Zory Shaposhnik
Division of Cardiology

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