ISH on slides

From:"Grant, Debra"

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Hi,
 
Does anyone have a protocol for performing ISH on cryo (oct) sectioned chick or mouse embryos on slides? What thickness do you section for the best staining? All suggestions would greatly be appreciated.
Thanks!
 
Debby Grant
Research Technician II
Histology Core Facility
Stowers Institute for Medical Research
1000 E. 50th Street
Kansas City, MO 64110
drg@stowers-institute.org
 
 

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Hi,
 
Does anyone have a protocol for performing ISH on cryo (oct) sectioned chick or mouse embryos on slides? What thickness do you section for the best staining? All suggestions would greatly be appreciated.
Thanks!
 
Debby Grant
Research Technician II
Histology Core Facility
Stowers Institute for Medical Research
1000 E. 50th Street
Kansas City, MO 64110
drg@stowers-institute.org
 
 
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