Hematoxylin blue in smooth muscle

From:Millicent Pope

Hello, everyone-  
I am having some trouble with H&E staining on a piece of canine
stomach.  There are patches of light blue showing up where it should be
pink.  I'm pretty sure it's not my staining protocol or technique
producing this artifact, as I have spent the last two weeks trying
everything I could to get this tissue uniformly pink.  As it stands now,
I am using Gill's hematoxylin, the nuclei are nicely stained, and there
is no hematoxylin background staining anywhere on the tissue escept
these light blue spots with dark blue nuclei.  Also, everything else in
the rack being stained at the same time is coming out nicely.  Can
anyone think of anything that would make patches of smooth muscle become

Milli Pope
Texas A&M University
VAPH Histology

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