(Fwd) skin IF

From:"Mcleod, H, Heather"

------- Forwarded Message Follows -------
Date:          Tue, 15 Apr 2003 13:00:42 +0200
From:          "Mcleod, H, Heather" 
Subject:       skin IF
To:            histonet@pathology.swmed.edu

Dear All
Thankyou for all the replies with regards to Indirect IF on skin 
A few questione were asked.
The disease process is Pemphigus.
Direct IF works well for linear Ig A and Ig G but intercellular 
staining is alway "iffy"
When we do direct IF, yes we do use frozen sections.
Can I use Rat oesopagus for the "antigen" source instead of the 
monkey oesophagus for the indirect IF?
Heather McLeod

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