FW: Luna's stain for elastin

From:"Monson, Frederick C.", p13 of the PDF has a recipe for
Gomori's AF in a discussion of how to combine it with Gomori's trichrome.
This is from an old Histo Logic issue, most of which are available in PDF
form at the sakura site.

Of course, Luna may have had a variant of the Gomori AF, but I am not aware
of it.


Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Mail Drop:  Geology
West Chester University
West Chester, PA, 19383
Phone/FAX:  610-738-0437 

-----Original Message-----
From: ncragg [mailto:n.cragg@epistem.co.uk]
Sent: Tuesday, April 22, 2003 6:05 AM
To: 'HistoNet Server'
Subject: Luna's stain for elastin

Does anyone have a protocol for Luna's stain for elastin?  I'm having 
trouble finding a protocol or even a paper which has the details.  I 
believe it is a modification of Luna's technique by Kligman.  If anyone has 
a copy of Kligman's paper "Luna Technique.  A beautiful stain for  elastin" 
Am J Dermatopathol 1981 Summer; 3(2): 199-201, I would be very grateful if 
you could pass it on.

Thank you in anticipation - I know Histonet won't let me down!  After hours 
of trawling the internet and histology books, there is only one sure fire 
way of getting the answer.

Kind regards,

Nicola Cragg
Epistem Ltd.
Manchester, UK

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