Hi,

 

I have a colleague interested in staining cytospun cells for Alkaline
phosphatase to localize it in her cells.  Then to analyze it using
confocal microscopy.  Does anyone have any advice on how to fix the
cells and a suitable Ab or kit?  She has tried the ELF 97 kit from
molecular probes with no success.

 

Many thanks in advance for the help.

 

Phil

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Philip Bergin

Göteborg University

Department of Medical Microbiology and Immunology

Box 435, SE-405 30 Göteborg, Sweden

Phone: +46-31-3424471

Fax: +46-31-826976