skin IF

From:"Mcleod, H, Heather"

Dear All
Thankyou for all the replies with regards to Indirect IF on skin 
A few questione were asked.
The disease process is Pemphigus.
Direct IF works well for linear Ig A and Ig G but intercellular 
staining is alway "iffy"
When we do direct IF, yes we do use frozen sections.
Can I use Rat oesopagus for the "antigen" source instead of the 
monkey oesophagus for the indirect IF?
Heather McLeod

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