In immunohistochemistry "who is the first"?

Pre-treatment (HIER or enzymatic digestion) of tissues and after hydrogen
peroxide to eliminate endogenous activity

or

Hydrogen peroxide to eliminate endogenous activity and after pretreatment
(HIER or enzymatic digestion) of tissues


I already see both procedures. Is not important the order?

With compliments

* * * * * * * * * * * * * * * * * * * * * * * * *
Fernando Capela e Silva
Laborat#243#rio de Biologia da Conserva#231##227#o
Departamento de Biologia
Universidade de ...vora
Apartado 94
7002-554 ...vora
PORTUGAL

Phone: +351-266 760 800
Fax: +351-266 711 231
Email: fcs@uevora.pt

http://evunix.uevora.pt/~fcs/FernandoCapelaSilva.htm