indirect skin if

From:"Mcleod, H, Heather"

Dear Histonetters

We do direct IFskin bx's, now the dermatologists would like indirect 
IF on selected cases.  While I have managed to split the normal 
skin tissue pretty well, the results are not encouraging in that all 
or very little flouresces.  What should one ideally be using for 
normal skin?  We have Dako fitc conjugated antibodies ............... 
what sort of dilutions should one be using? I stuck to same dilutions 
that we use for direct IF.  And the blister fluid or patient serum  I 
used neat, should they be diluted?  ANY help and suggestiopns would 
be appreciated.


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