To the Histonetter who was wondering wether to use the hydrogen 
peroxide before or after the antibody incubation , I use it before the 
incubation but have read that it can mask or destroy some antigen 
sites. So if you're having problems , try doing it directly after the 
incubation.
Margaret Horne 
Dept. of A&P,
Atlantic Veterinary College, U.P.E.I.
550 University Ave., Charlottetown,
P.E.I.,  C1A 4P3 
Canada