(hot antibody and retrieval solutions with paraffin emu
Has anyone tried a hot antibody technique and how well did it work?
I came across a method using an antibody incubation at 60C on
gluteraldehyde -f ixed , epoxy-embedded sectons , as well as
formalin fixed parafin -embedded tissue.
Also , there is a reference in another paper for using retrieval
solutions that have paraffin emulsifiers in them. So it is a one step
deparaffin and hot antigen retieval. Supposed to save money as
well as time. Time - I can see, money- I am checking out. I am
wondering how well they work. Has anyone out there used them?
Thanks , Margaret
------- End of forwarded message -------
Dept. of A&P,
Atlantic Veterinary College, U.P.E.I.
550 University Ave., Charlottetown,
P.E.I., C1A 4P3
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