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Hi all,
 
I am preparing mouse embryo in gelatin for vibratome and cryostat sectioning. Are there any differences in preparing the two gelatin molds for vibratome and for cryostat sectioning? I prepared the embryo in gelatin for cryo sections the same way I prepared the other embryo for vibratome sectioning and I had many holes in the embryo sections as the same type of holes in the outer part of the gelatin section. All protocols would be appreciated as I am working with ISH stained and non ISH stained mouse embryo, if this makes a difference.
 
Thanks!
 
Debby Grant
Research Technician II
Histology Core Facility
Stowers Institute for Medical Research
1000 E. 50th Street
Kansas City, MO 64110
drg@stowers-institute.org
 
 

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Hi 
all,
 
I am preparing mouse 
embryo in gelatin for vibratome and cryostat sectioning. Are there any 
differences in preparing the two gelatin molds for vibratome and for cryostat 
sectioning? I prepared the embryo in gelatin for cryo sections the same way I 
prepared the other embryo for vibratome sectioning and I had many holes in the 
embryo sections as the same type of holes in the outer part of the gelatin 
section. All protocols would be appreciated as I am working with ISH stained and 
non ISH stained mouse embryo, if this makes a difference.
 
Thanks!
 
Debby Grant
Research Technician II
Histology Core Facility
Stowers Institute for Medical Research
1000 E. 50th Street
Kansas City, MO 64110
drg@stowers-institute.org
 
 

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