Thanks to you listers who replied to yesterday's query about the dog
teeth. I think I've settled on cutting the teeth with a diamond saw
after a short fixation, then going from there.
Now I have another question. I have several mouse fetuses to process,
and I could use some advice on processing times. These little guys are
between 2.25 and 2.5 cm long, and have diameters between .75 and 1 cm.
Our standard reagents on the processor are (in this order) 70% EtOH, 2
stations of 80% EtOH, two stations of 95% EtOH, two stations of absolute
alcohol, and three stations of xylene substitute. Can anyone suggest
some reagent times? I don't have much experience with fetal tissue, and
I am afraid of overprocessing.
Texas A&M University
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