Re: "Who is the first"

From:IHC World

RE: "Who is the first"
In theory, blocking of endogenous peroxidase activity can be applied at any step BEFORE incubation of HRP-conjugated antibody. I usually do the blocking step after HIER/PIER, but have been testing it before HIER/PIER. No difference was observed. So I think the key is to be consistent cross all experiments.
IHC World
----- Original Message -----
From: Tony Henwood
To: 'Fernando Capela e Silva' ;
Sent: Wednesday, April 09, 2003 4:43 PM
Subject: RE: "Who is the first"

We block endogenous peroxidase (Methanol-H2O2) before HIER or enzyme retrieval

Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318

-----Original Message-----
From: Fernando Capela e Silva []
Sent: Wednesday, 9 April 2003 18:31
Subject: "Who is the first"

In immunohistochemistry "who is the first"?

Pre-treatment (HIER or enzymatic digestion) of tissues and after hydrogen
peroxide to eliminate endogenous activity


Hydrogen peroxide to eliminate endogenous activity and after pretreatment
(HIER or enzymatic digestion) of tissues

I already see both procedures. Is not important the order?

With compliments

* * * * * * * * * * * * * * * * * * * * * * * * *
Fernando Capela e Silva
Laborat#243#rio de Biologia da Conserva#231##227#o
Departamento de Biologia
Universidade de ...vora
Apartado 94
7002-554 ...vora

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