Re: human Ab on human

From:Carrie Kyle-Byrne

there is a difference between a true human anti-human antibody and a
humanized antibody.  i'm assuming that your antibody is a humanized murine
monoclonal.  humanized antibodies are a fusion of human Fab and murine Fc
(there's more to it but this is a simplified explanation).  the only part of
the antibody that is human is the part that will bind to the tissue.  the
part of the antibody that you will be binding secondary to is MOUSE.
therefore, you need to use an anti-mouse secondary.
Carrie Kyle-Byrne, BHS, HT(ASCP)
Assoc. Research Scientist II
Antibody Core Lab
Signal Transduction Research

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----- Original Message -----
From: "joyce judge" 
Sent: Friday, April 11, 2003 5:00 AM
Subject: human Ab on human

> I have gone through the histonet archives to look for answers regarding
> Human Ab on Human tissue. I was wondering how people have  resolved this
> issue.  I will need to do IHC on a "humanized" antibody in human tissue
> we can not biotinylate or conjugate the primary at all.  Can anyone offer
> any help?
> Joyce Judge
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