Re: another query about mouse embryo's
From: | "t.hacker@har.mrc.ac.uk" |
Date sent: Tue, 01 Apr 2003 09:00:47 -0600
From: Millicent Pope
Subject: another query
To: histonet@pathology.swmed.edu
> Now I have another question. I have several mouse fetuses to process,
> and I could use some advice on processing times. These little guys are
> between 2.25 and 2.5 cm long, and have diameters between .75 and 1 cm.
Millicent,
at that size I would assume these mice are about 19 -19.5 dpc
( or you have in fact got rats!). At this age we would usually bisect the fetus
sagittally ( by hand using a microtome blade) after a few hours fixation,
then further fix for 24 hours.We also decal for 48hours in EDTA prior to
processing with a standard overnight process. There is no evidence of "over-
processing".
Terry.
Terry Hacker,
Head of Histopathology and Electron Microscopy
Medical Research Council,
Harwell,
Didcot,
Oxfordshire, OX11 ORD
01235 841128
Fax 01235 834776
e-mail t.hacker@har.mrc.ac.uk
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