Behnaz, our lab use to order Zeus Fixative, but the cost and date of
expiration was always a problem. Below is what our EM lab prepares for
immunofluorescent studies on renal biopsy, and gives out to our clientel.
Hope this helps. Teresa

Reference to Michels Transport Media (alias Zeus Fixative):
Michel M.D., Yorman; Niler Phd, David, Kathy M.T.:  Preservation of tissue
fixed immunoglobulins in skin biopsies of patients with lupus. J. Invest.
Derm. 59:449-452 (1973).

CALCULATIONS:
1.  Prepare a solution of 0.1M K citrate and ph to 7.2 - ph 7.4
	K-citrate 32.44 grams
	Distilled water 100.00 ml
2.  Prepare a solution of 0.1M MgS04 7H2
	MgS04 7H2 2.40 grams
	Distilled water 100.00 ml
3.  Prepare a solution of 0.1M N-ethyl maleimide
	N-ethyl maleimide 1.2521 grams
	Distilled water 100.00 ml
4. Prepare a BUFFER Solution
		0.1M K-citrate 		2.5ml
		0.1M MgS04 7H2 		5.0ml
		0.1M N-ethyl maleimide 	5.0ml
		Distilled water to make 100.00 ml
5. The prepared Michel's Transport Media will ph to 7.4
		(NH4)S04 55 grams
		Prepared BUFFER Solution 100.00 ml
A low frequency of false negative will result if biopsy is held in Michel's
Transport Media for over 5 days.

I WAS WONDERING IF ANY ONE HAS THE MAGIC FORMULA FOR THE "  ZEUS " FIXATIVE
. WE USE IT TO TRANSPORT TISSUE. THANKS