Re: Tissue processing for GP Brains

From:Gayle Callis

The animal tissue processing manual is intended to be guidelines although
one can certainly try the protocols as written.  You may have to see what
is in the manual, then tweak the protocol for larger tissue.  

You did not give your protocol for guinea pig brains - it would help plus
the dimensions on the brain.  Larger size tissues will need longer
processing times.  


At 07:59 PM 4/11/2003 -0400, you wrote:
>  processing brain tissue.
>  edge of the tissue being dry.
> I have a Shandon Hypercenter XP processor and we use Microclear.
> Sometimes the are brains are post fixed in Potassium Dichromate for about
>2.5hrs then rinsed well in running water after being in 10% NBF. Sometimes
>we can't postfix our tissue and brain was only in 10% NBF. Sometimes are
>brains are profused and sometimes not. This always depend on the study that
>is ordered.
> If you would just like for me to call you I can't do this to.
> Thank you for any help you can give me.
> Sandi Miller HT (ASCP),RALAT
> United States Army Medical Research Institute of Chemical Defense
> (410)436-1967 
Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
S. 19th and Lincoln St
Bozeman MT 59717-3610

406 994-6367 (lab with voice mail)
406 994-4303 (FAX)


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