Re: Quick question about Fluoro-Jade B...
Michael A Kuluz wrote:
> What part of the neuron does this substance stain to show degenerating
> neurons? I have searched high and low and have yet to learn this "trade
> secret". Thanks in advance...
The published papers about this secret substance
describe it as a fluorescent anionic xanthene dye.
Eosin is such a dye; so is fluorescein. Such dyes
bind most strongly to basic proteins, which seem
to occur at high concentrations in the nuclei and
cytoplasm of dead cells.
Here are a few refs that might help you to decide
what to do.
Acidophilia of dead neurons:
Kiernan JA, Macpherson CM, Price A, Sun T (1998)
A histochemical examination of the staining of
kainate-induced neuronal degeneration by anionic
dyes. Biotech. Histochem. 73: 244-254. (This includes
references to earlier papers on the subject.)
"Fluoro-jade" for dead neurons:
Schmued LC, Albertson C, Slikker W (1997) Fluoro-Jade:
a novel fluorochrome for the sensitive and reliable
histochemical localization of neuronal degeneration.
Brain Res. 751: 37-46.
Schmued LC, Hopkins KJ (2000) Fluoro-Jade: novel
fluorochromes for detecting toxicant-induced neuronal
degeneration. Toxicologic Pathology 28: 91-99.
"Fluoro-jade" is not specific for dead neurons:
Colombo JA, Puissant VI (2002) Fluoro jade stains early
and reactive astroglia in the primate cerebral cortex.
J. Histochem. Cytochem. 50: 1135-1137.
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London, Canada N6A 5C1
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