Nobody else has posted an answer to this one, so
here you are. The method is for paraffin sections
of formaldehyde-fixed CNS.
 
1. Treat hydrated sections with 4% iron alum, overnight, room
   temp.
2. Wash in water for 5 min.
3. Stain overnight, room temp, in a freshly prepared mixture of:
      10% alcoholic haematoxylin (well ripened) 10 ml
      Water                                     90 ml
      Sat. aq. lithium carbonate                 4 ml
    (Alternatively 3 hours at 56C)
4. Wash in water.
5. Immerse in 4% iron alum to remove much of the background
   colour.
6. Wash in water.
7. Immerse in borax-ferricyanide (1% borax, 1.25% K3Fe(CN)6 in
   water) until only the myelin sheaths remain stained
(blue-black).
8. Wash in water; Optionally counterstain with eosin;
   dehydrate, clear and cover.
 
(HC Cook 1974. Manual of Histological Demonstration Techniques.
London: Butterworths p.161-162. Cook gives a reference to
Loyez 1910, in a French journal.)
--
-------------------------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
   kiernan@uwo.ca
   http://publish.uwo.ca/~jkiernan/
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> Sarah Jones wrote:
> >
> > Greetings,
> >   I'm looking for a procedure for the Loyez method for Myelin staining
> > if anyone would like to share.  I have a few procedures gleaned off the
> > internet but they are for free floating sections.  Would the procedure
> > work on mounted sections as well?  I also have a reprint of an article,
> > but it is in German.  Would anyone on the Histonet care to translate the
> > staining procedure for me?   Danke, Sarah
> >
> > Sarah Jones HT(ASCP)
> > Dept. of Vet. Anatomy & Public Health
> > Histology Lab
> > Texas A&M University
> > College Station, TX 77843-4458
> > phone: 979-845-3177
> > fax:  979-458-3499
> > email: sjones@cvm.tamu.edu
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