Re: Low temperature HIER
Here's something similar.
Koopal SA, Coma MI, Tiebosch ATMG, Suurmeijer AJH (1998)
Low-temperature heating overnight in tris-HCl buffer pH 9 is a
good alternative for antigen retrieval in formalin- fixed
paraffin-embedded tissue. Applied Immunohistochemistry 6:
Their "low temperature" was 80C.
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London, Canada N6A 5C1
> Jeffrey Silverman wrote:
> I read a paper by Michel Margiotta and Jules Elias about low
> temperature HIER- using prolonged incubations in the same HIER
> buffers used for regular HIER but at 70 or 80 degrees C. It
> was in J of Histotechnol- anyone have the exact issue??
> Jeff Silverman
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