Hi Mary,
I used to have exactly the same problem. It was recommended to me to mount
from TBS pH 7.6 and not from distilled water, which tends to be on acidic
side. I never proved this to myself, since I had changed to DAB by then, but
it's worth a try.

Katri

Katri Tuomala
Department of Pathology
St Joseph's Health Care
Hamilton, Ontario, Canada

----- Original Message -----
From: "Mary Bryhan" 
To: 
Sent: Thursday, April 03, 2003 10:23 AM
Subject: IHC - Hematoxylin fading


> Does anyone have any problems with the hematoxylin fading from their IHC
sections after coverslipping?  We use Mayer's hematoxylin after AEC and
mount with Dako's Glycergel.
>
> Mary Bryhan
>
>