HistoMouse DAB Kit (from Zymed) is good for all
mouse-on-mouse staining.

Joe
SFSU



--- Geoff McAuliffe  wrote:
>     I have used anti-F4/80 on mouse CNS fixed with
> 4% paraformaldehyde 
> perfusion, snap frozen and cut at 20 microns on a
> sliding microtome. I 
> ran the primary antibody at 1:1000 or 1:2000 all
> night at room temp. on 
> a shaker table (very gentle agitation) then used a
> Vector ABC Elite kit 
> and DAB intensified with either nickel + cobalt or
> Vector's "Intense" 
> reagent.  Beautiful results.
>     Clint Lincoln on this list has done F4/80 on
> FFPE sections after 
> enzyme treatment.
> 
> Geoff
> 
> Sylene.Goodwin@UCHSC.edu wrote:
> 
> >Has anyone used this on frozen mouse tissue? 
> Particularly mouse kidney?  What type of fixation
> did you use?  Caltag's technical support and
> references are not exceptionally helpful.
> >
> >Thank-you!
> >
> >Sylene
> >
> >  
> >
> 
> -- 
> --
> **********************************************
> Geoff McAuliffe, Ph.D.
> Neuroscience and Cell Biology
> Robert Wood Johnson Medical School
> 675 Hoes Lane, Piscataway, NJ 08854
> voice: (732)-235-4583; fax: -4029 
> mcauliff@umdnj.edu
> **********************************************
> 
> 
> 
> 


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