Re: Bleeding Eosin
if you are using a xylene
substitute on the back end of the staining line, it might have too much water in
it. You might have to add another alcohol. Is humidity a
absorb some water, but xylene substitutes are not as forgiving.
Joe Nocito, BS, HT (ASCP)
Pathology Reference Lab
San Antonio, TX
----- Original Message -----
Sent: Wednesday, April 02, 2003 1:14
Subject: Bleeding Eosin
On a batch of slides from a couple of days ago, I noticed that the eosin
on the H&E's had bled from the tissue sections well after
coverslipping. I've never seen this before and any advice you may have
would be greatly appreciated.
Thank you in advance for your help.
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