Re: Bleeding Eosin

From:Joe Nocito

if you are using a xylene substitute on the back end of the staining line, it might have too much water in it.  You might have to add another alcohol.  Is humidity a problem.
    Xylene can absorb some water, but xylene substitutes are not as forgiving.
Joe Nocito, BS, HT (ASCP) QIHC
Histology Supervisor
Pathology Reference Lab
San Antonio, TX
----- Original Message -----
From: Louri Caldwell
Sent: Wednesday, April 02, 2003 1:14 PM
Subject: Bleeding Eosin

Hi everyone,
On a batch of slides from a couple of days ago, I noticed that the eosin on the H&E's had bled from the tissue sections well after coverslipping.  I've never seen this before and any advice you may have would be greatly appreciated.
Thank you in advance for your help.
Louri Caldwell

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