RE: hydrogen peroxide
I always use the h2o2 block after the primary antibody.
From: Margaret Horne [mailto:firstname.lastname@example.org]
Sent: Wednesday, April 09, 2003 11:50 AM
To: HistoNet Server
Subject: hydrogen peroxide
To the Histonetter who was wondering wether to use the hydrogen
peroxide before or after the antibody incubation , I use it before the
incubation but have read that it can mask or destroy some antigen
sites. So if you're having problems , try doing it directly after the
Dept. of A&P,
Atlantic Veterinary College, U.P.E.I.
550 University Ave., Charlottetown,
P.E.I., C1A 4P3
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