RE: (hot antibody and retrieval solutions with paraffin emu

From:Patsy Ruegg

never used hot antibodies but I have used the retrieval reagents that
deparaffinize with the BioCare Decloaker (pressure cooker)  Reveal and Borg
(high ph) they are called.  They work well in my experience.  I have done
studies comparing their use to other retrieval reagents that require
deparaffination before use and had no differences in final results.

-----Original Message-----
From: Margaret Horne []
Sent: Tuesday, April 01, 2003 10:31 AM
To: HistoNet Server
Subject: (hot antibody and retrieval solutions with paraffin emu

Has anyone tried a hot antibody technique and how well did it work?
I came across a method using an antibody incubation at 60C on
gluteraldehyde -f ixed , epoxy-embedded sectons , as well as
formalin fixed parafin -embedded tissue.

 Also , there is a reference in another paper for using retrieval
solutions that have paraffin emulsifiers in them. So it is a one step
deparaffin and hot antigen retieval.  Supposed to save money as
well as time. Time - I can see, money- I am checking out. I am
wondering how well they work. Has anyone out there used them?

                              Thanks , Margaret
------- End of forwarded message -------
Margaret Horne
Dept. of A&P,
Atlantic Veterinary College, U.P.E.I.
550 University Ave., Charlottetown,
P.E.I.,  C1A 4P3

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