RE: biotin containing tissues
Add to the list of endogenous biotin containing tissues: most human
epithelial tissues, human cardiac myocytes and rat spleen.
Realize that the more sensitive/efficient your (strept)avidin-biotin-based
detection system is, the stronger the visualization of endogenous biotin
Chris van der Loos
Dept. of Cardiovascular Pathology
Academical Medical Center
Amsterdam - The Netherlands
Linda Sebree wrote:
>Date: 10 Apr 2003 10:39:26 -0600
>From: "Sebree Linda A."
>Subject: biotin containing tissues
>Does anyone out there have a good reference or list of tissues containing
>endogenous biotin? We don't like to put avidin-biotin block on our staining
>procedures unless the tissue requires it. We're aware of liver and kidney
>there are other tissue types that also contain biotin to a lesser degree.
>Thanks for your help.
>Linda A. Sebree, HT(ASCP)
>University of Wisconsin Hospital & Clinics
>600 Highland Ave.
>Madison, WI 53792
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