DakoCytomation, has a new detection kit which is biotin free.  It is
Envision + Dual link.  The titers can be extended out further because, it is
usually 10 times stronger than there other detection kits.  I really like
using it because not only does it save time and money but, it is much easier
to teach someone how to do immuno's.  The following is there web site if you
would like more info.  Hope you find this helpful.

www.dakocytomation.com
       

-----Original Message-----
From: Fred Underwood [mailto:funderwood@mcohio.org]
Sent: Thursday, April 10, 2003 9:55 AM
To: la.sebree@hosp.wisc.edu; histonet@pathology.swmed.edu
Subject: RE: biotin containing tissues


You can add brain to that list.  A possible reference is , Appl.
Immunohistochem: issue 5, 1997.  I have swithced to a non-biotin
amplification kit from Zymed.  
1-800-874-4494, www.zymed.com.  Additional info on this kit is in JOH volum
23, #4 December 2000.
 
Fred

	-----Original Message-----
From: "Sebree Linda A."  
Sent: Thursday, April 10, 2003 12:29 PM
To: Histonet 
Subject: biotin containing tissues



	Does anyone out there have a good reference or list of tissues
containing endogenous biotin?  We don't like to put avidin biotin block on
our staining procedures unless the tissue requires it.  We're aware of liver
and kidney but there are other tissue types that also contain biotin to a
lesser degree.  Thanks for your help.

	Linda A. Sebree, HT(ASCP) 
University of Wisconsin Hospital & Clinics 
IHC/ISH Laboratory 
A4/204 2472 
600 Highland Ave. 
Madison, WI 53792 
(608)265 6596 
FAX: (608)262 7174 


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