It's called Maleimide Buffer, and we use to do one rinse for 15 min.

Good luck,

Juan C. Gutierrez, HT(ASCP)
Histology Supervisor
Christus Santa Rosa Healthcare
(210)704-2533
Juan_Gutierrez@srhc.iwhs.org


-----Original Message-----
From: Kathleen A Spencer [mailto:KSPENCER@UTMEM.EDU]
Sent: Wednesday, April 09, 2003 1:19 PM
To: Behnaz Sohrab; Teresa Flores
Cc: histonet
Subject: RE: ZEUS FIXATIVE


I once worked in a lab where we received renal biopsy's in Michel's 
transport medium, but before we could freeze them for cryo-sectioning we 
had to take them through several rinses in Michel's rinse or was it called 
wash buffer? I can't remember. Does anyone have a clue? I think this is a 
very necessary step.

Kathleen Spencer


>===== Original Message From Teresa Flores  
=====
>Behnaz, our lab use to order Zeus Fixative, but the cost and date of
>expiration was always a problem. Below is what our EM lab prepares for
>immunofluorescent studies on renal biopsy, and gives out to our clientel.
>Hope this helps. Teresa
>
>Reference to Michels Transport Media (alias Zeus Fixative):
>Michel M.D., Yorman; Niler Phd, David, Kathy M.T.:  Preservation of tissue
>fixed immunoglobulins in skin biopsies of patients with lupus. J. Invest.
>Derm. 59:449-452 (1973).
>
>CALCULATIONS:
>1.  Prepare a solution of 0.1M K citrate and ph to 7.2 - ph 7.4
>	K-citrate 32.44 grams
>	Distilled water 100.00 ml
>2.  Prepare a solution of 0.1M MgS04 7H2
>	MgS04 7H2 2.40 grams
>	Distilled water 100.00 ml
>3.  Prepare a solution of 0.1M N-ethyl maleimide
>	N-ethyl maleimide 1.2521 grams
>	Distilled water 100.00 ml
>4. Prepare a BUFFER Solution
>		0.1M K-citrate 		2.5ml
>		0.1M MgS04 7H2 		5.0ml
>		0.1M N-ethyl maleimide 	5.0ml
>		Distilled water to make 100.00 ml
>5. The prepared Michel's Transport Media will ph to 7.4
>		(NH4)S04 55 grams
>		Prepared BUFFER Solution 100.00 ml
>A low frequency of false negative will result if biopsy is held in Michel's
>Transport Media for over 5 days.
>
>I WAS WONDERING IF ANY ONE HAS THE MAGIC FORMULA FOR THE "  
ZEUS " FIXATIVE
>. WE USE IT TO TRANSPORT TISSUE. THANKS