RE: Orcein staining for elastic fibres in skin
I personally would not recommend that you use Orcein as in my hands it has not proved to be effective in showing fine elastic fibers. I would recommennd Hart's modification of Weigert's elastin stain.
From: ncragg [mailto:email@example.com]
Sent: Wed 4/2/2003 2:04 AM
To: 'HistoNet Server'
Subject: Orcein staining for elastic fibres in skin
Please could anyone help me on which protocol to use for orcein staining
for elastic fibres in skin?
I have several protocols and I'm not sure which one to use. I have a basic
protocol (stain rehydrated sections with orcein, dehydrate and mount) and
protocols which involve prior stages of oxidising with potassium
permanganate, bleaching with oxalic acid and immersion in periodic acid. I
am aware that orcein also stains for Hep B antigen in liver tissue,
therefore, I wasn't sure whether these extra steps are something to do with
the tissue being stained.
I need to stain frozen sections of skin and therefore, was also wondering
whether air drying and fixing in 10% formalin, would be OK for this
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