RE: HIER

From:Scott Mordue

Make sure you are using SuperFrost slides or pretreat
your slides with HistoGrip Solution to provide strong
adhesion between the tissue and slide.

Scott
CSU

--- "Sebree Linda A."  wrote:
> I don't know what your heating method is but you
> might want to try a gentler one than you're using. 
> Try a steamer or pressure cooker or if you don't
> have those, try bringing your slides and buffer just
> up to a simmer in a microwave, then transfer them to
> a 93-95 degree water bath.  The buffer won't boil
> this way and you may have better luck with tissue
> adherence.
> 
> Linda A. Sebree, HT(ASCP)
> University of Wisconsin Hospital & Clinics
> IHC/ISH Laboratory
> A4/204-2472
> 600 Highland Ave.
> Madison, WI 53792
> (608)265-6596
> FAX: (608)262-7174
> 
> 
> -----Original Message-----
> From: Fernando Capela e Silva [mailto:fcs@uevora.pt]
> Sent: Thursday, April 10, 2003 2:45 AM
> To: HISTONET@pathology.swmed.edu
> Subject: HIER
> 
> 
> I use Citrate buffer (PH 6.0) at 95-98#186# for 10
> min as HIER. However this
> method cause severe damage in some sections and
> other are completly lost. In
> a paper the authors used 14% EDTA at 65#186# C
> overnight.
> 
> I would like if someone help me about this problem
> and if I is a good choice
> using citrate incubation overnight .
> 
> With compliments.
> 
> 
> * * * * * * * * * * * * * * * * * * * * * * * * *
> Fernando Capela e Silva
> Laborat#243#rio de Biologia da Conserva#231##227#o
> Departamento de Biologia
> Universidade de ...vora
> Apartado 94
> 7002-554 ...vora
> PORTUGAL
> 
> Phone: +351-266 760 800
> Fax: +351-266 711 231
> Email: fcs@uevora.pt
> 
> http://evunix.uevora.pt/~fcs/FernandoCapelaSilva.htm
> 
> 
> 
> 


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