Make sure you are using SuperFrost slides or pretreat
your slides with HistoGrip Solution to provide strong
adhesion between the tissue and slide.
--- "Sebree Linda A." wrote:
> I don't know what your heating method is but you
> might want to try a gentler one than you're using.
> Try a steamer or pressure cooker or if you don't
> have those, try bringing your slides and buffer just
> up to a simmer in a microwave, then transfer them to
> a 93-95 degree water bath. The buffer won't boil
> this way and you may have better luck with tissue
> Linda A. Sebree, HT(ASCP)
> University of Wisconsin Hospital & Clinics
> IHC/ISH Laboratory
> 600 Highland Ave.
> Madison, WI 53792
> FAX: (608)262-7174
> -----Original Message-----
> From: Fernando Capela e Silva [mailto:email@example.com]
> Sent: Thursday, April 10, 2003 2:45 AM
> To: HISTONET@pathology.swmed.edu
> Subject: HIER
> I use Citrate buffer (PH 6.0) at 95-98#186# for 10
> min as HIER. However this
> method cause severe damage in some sections and
> other are completly lost. In
> a paper the authors used 14% EDTA at 65#186# C
> I would like if someone help me about this problem
> and if I is a good choice
> using citrate incubation overnight .
> With compliments.
> * * * * * * * * * * * * * * * * * * * * * * * * *
> Fernando Capela e Silva
> Laborat#243#rio de Biologia da Conserva#231##227#o
> Departamento de Biologia
> Universidade de ...vora
> Apartado 94
> 7002-554 ...vora
> Phone: +351-266 760 800
> Fax: +351-266 711 231
> Email: firstname.lastname@example.org
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