The step before serum blocking solution.
 
Fred

	-----Original Message-----
From: Fernando Capela e Silva  
Sent: Monday, April 14, 2003 2:19 PM
To: 
Subject: Avidin/Biotin blocking



	In general immunohistochemistry protocol where should I use avidin/biotin 
blocking? 

	1. Peroxidase quenching solution 
2. Pre treatment/Antigen Retrieval 
3. Serum blocking solution (10% non imune serum) 
4. Primary antibody 
5. Secondary Antibody 
6. Streptavidin/Peroxidase 
7. Substrate/Chromogen 
8. Counterstaining 
9. Mounting 

	Best Regards 

	Fernando 
* * * * * * * * * * * * * * * * * * * * * * * * * 
Fernando Capela e Silva 
Laborat#243#rio de Biologia da Conserva#231##227#o 
Departamento de Biologia 
Universidade de ...vora 
Apartado 94 
7002 554 ...vora 
PORTUGAL 

	Phone: +351 266 760 800 
Fax: +351 266 711 231 
Email: fcs@uevora.pt 

	http://evunix.uevora.pt/~fcs/FernandoCapelaSilva.htm