Orcein staining for elastic fibres in skin
Please could anyone help me on which protocol to use for orcein staining
for elastic fibres in skin?
I have several protocols and I'm not sure which one to use. I have a basic
protocol (stain rehydrated sections with orcein, dehydrate and mount) and
protocols which involve prior stages of oxidising with potassium
permanganate, bleaching with oxalic acid and immersion in periodic acid. I
am aware that orcein also stains for Hep B antigen in liver tissue,
therefore, I wasn't sure whether these extra steps are something to do with
the tissue being stained.
I need to stain frozen sections of skin and therefore, was also wondering
whether air drying and fixing in 10% formalin, would be OK for this
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