Michel's transport medium (Re: ZEUS FIXATIVE)
In case you want to make your own Michel's transport
medium, instructions can be found on the web at
This FAQ answer (by Bob Richmond) also explains how
to use the stuff, and provides a reference to Michel's
paper. The mixture has several ingredients (I could
speculate about what they all do, but won't.)
The liquid is not a fixative.
Bob Richmond notes that Zeus Medium is "probably
similar." Unless it's declared to be definitely
the same as Michels's, it might be prudent to make
your own. The work involved is, for someone
inexperienced, about half an hour of weighing,
measuring and pH checking. Anyone who does these
jobs regularly could make any volume of Michel's
medium in 15 minutes.
Juan Guttierez' point about "expires rather fast"
is an important one. "Expiration" for a citrate buffer
containing ammonium and magnesium ions (that's what
Michel's medium is) means infection by bacteria and
fungi derived from airborne spores.
A pre-made solution of this kind _ought_ to be sold
in small, sterilized batches, which should be as good
as new until opened. (I don't know if this is how
such such solutions are sold.)
If you mix your own it will start collecting and
feeding microorganisms right away. In my lab citrate
buffers become visibly mouldy in about a month.
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London, Canada N6A 5C1
"Gutierrez, Juan" wrote:
> Yes, it's called Michel's fixative, and you can get it from Poly Scientific
> R&D Corp.
> Their number is 1-800-645-5825.
> Catalog #s2168. It's easier to buy it than to make it. It also expires
> rather fast, so dont order too much.
> Hope this helps,
> Juan C. Gutierrez
> Histology Supervisor
> Christus Santa Rosa Healthcare
> -----Original Message-----
> From: Behnaz Sohrab [mailto:SohrabB@wmmcpo.ah.org]
> Sent: Tuesday, April 08, 2003 12:22 PM
> To: firstname.lastname@example.org
> Subject: ZEUS FIXATIVE
> I WAS WONDERING IF ANY ONE HAS THE MAGIC FORMULA FOR THE " ZEUS " FIXATIVE .
> WE USE IT TO TRANSPORT TISSUE.
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