In general immunohistochemistry protocol where should I use avidin/biotin
blocking?

1. Peroxidase quenching solution
2. Pre-treatment/Antigen Retrieval
3. Serum blocking solution (10% non-imune serum)
4. Primary antibody
5. Secondary Antibody
6. Streptavidin/Peroxidase
7. Substrate/Chromogen
8. Counterstaining
9. Mounting

Best Regards

Fernando
* * * * * * * * * * * * * * * * * * * * * * * * *
Fernando Capela e Silva
Laborat#243#rio de Biologia da Conserva#231##227#o
Departamento de Biologia
Universidade de ...vora
Apartado 94
7002-554 ...vora
PORTUGAL

Phone: +351-266 760 800
Fax: +351-266 711 231
Email: fcs@uevora.pt

http://evunix.uevora.pt/~fcs/FernandoCapelaSilva.htm