sections surface relief - incredibly irregular

From:=?ISO-8859-1?Q?Anne_Skakkeb=E6k_Jensen?=

 
We need 3um thick sections of soft tissues for measurement of microelastic
properties by use of ultrasound. We already tried the usual procedure with
paraffin and the microtome, but measurements (profilometry and laser
techniques) have shown that these sections are indeed not of the intended
thickness, and EVEN WORSE, the surfaces are incredibly irregular, which our
ultrasound does not permit.

So the surface relief is our problem. We could use harder embedding
materials like plastics etc., but those will likely change the properties
that we are interested in.

Does anyone have good suggestions as how to prepare thin sections of soft
tissue without surface relief and without damaging the elastic properties of
the tissue???

Yours sincerely
Anne Skakkebęk Jensen
Institute of Experimental Clinical Research
Skejby University Hospital
Aarhus, Denmark



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From: HistoNet Server
To: Anne#u#Skakkeb#190#k#u#Jensen
Sent: 30-04-2002 10:47
Subject: re: subscribe

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es




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