sections storage (2)

From:natalya karp

Dear Histonetters,

(I posted the below message before, but I got no
responses (probably of the timing was wrong), so I'm
trying again.)

I have been doing TUNEL staining on Peyer's patches of
the small intestine and spleen. Tissues are derived
form mice treated with a cytotoxic drug, with the
purpose of evaluation of apoptosis as a marker of the
drug's toxicity.

My questions are: 
1)What are the rules for the storage of paraffin
blocks?  Should the blocks be stored in the
refrigerator or at the room temperature?
2)For how long the sections can be stored at the room
temperature before the staining? 
3)If the sections are stored at the room temperature
for several month, could one expect the increase in
the number of apoptotic cells due to spontaneous DNA

Any comments and responses will be greatly

Natalya Karp, M.D.
McGill University, Dept. of Human Genetics
Montreal, Quebec, Canada

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