From: | "April E. Monchik" |
Hi, I'm trying to process, embed, and section in paraffin cultured cells called HL60s. I am having trouble getting the cells to pellet cohesively. Does anyone have any ideas on how to section a cell pellet in paraffiin? I understand I need to embed the pellet in gelatin first, but what percentage is used? Any helpful hints or ideas would be greatly appreciated. - April