hematoxylin counterstain on IHC


Has anyone ever had a problem getting Mayer's hematoxylin into IHC sections,
particularly on an automated stainer; but also occasionally with manual ones
too. The immunohistochemistry has worked well. This has started happening here
recently on some but not all sections; we have tried a new batch of hematoxylin
with no success.
Ronnie Houston
Regional Histology Operations Manager
Bon Secours Health Partners Laboratories
5801 Bremo Road
Richmond, VA 23226

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