help - IHC

From:"Mcleod, H, Heather"

Dear Histonetters
I am trying, without much success to stain ffpe sections with the 
following antibodies
(1) Integrin alpha-6 (BQ16) Santa Cruz sc-13542 Mouse monoclonal and 
(2) Fibulin-1 (C-16) Santa Cruz sc-8675 Goat polyclonal
I'll be grateful for advice from anyone who has used these ab's on 
ffpe material.
I am told that all SC antibodies are optimised for / on  frozen 
sections.  What is the trick to getting such antibodies to stain 
fixed material?  
Heather McLeod (GSH / UCT)

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