c-kit (CD117)

From:"Klassen, Shannon SDH"

Can someone out there in histoland help me get this antibody working -
Novocastra ckit clone T595 - I'm not getting membrane staining - some
cytoplasmic and some almost appears nuclear. I've tried a variety of
different tumors - GIST, melanoma, breast , normal skin and haven't had any
Down to 1\10 with antigen retrieval in citrate buffer in microwave.

Shannon Klassen
IHC Tech
Saskatoon Health District
Saskatoon, Canada

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