Re: sections storage (2)
For what it's worth,my understanding is that once tissue is sufficiently
fixed all cellular activity should have been arrested and thereafter
changes are caused by subsequent processing i.e.shrinkage,therefore once
the tissue has been sucessfully embedded into wax it is safe to store
them almost indefinitely (I don't know about countries with extreme
temperatures) at room temperature.I am quite prepared to be corrected if
I am wrong about this.Also, I believe that it is quite safe to store the
slides already cut from wax blocks.
Hope this helps!
Histology/Anatomy/Biomedical Sciences Division/Uni.of Akld./N.Z.
natalya karp wrote:
> Dear Histonetters,
> (I posted the below message before, but I got no
> responses (probably of the timing was wrong), so I'm
> trying again.)
> I have been doing TUNEL staining on Peyer's patches of
> the small intestine and spleen. Tissues are derived
> form mice treated with a cytotoxic drug, with the
> purpose of evaluation of apoptosis as a marker of the
> drug's toxicity.
> My questions are:
> 1)What are the rules for the storage of paraffin
> blocks? Should the blocks be stored in the
> refrigerator or at the room temperature?
> 2)For how long the sections can be stored at the room
> temperature before the staining?
> 3)If the sections are stored at the room temperature
> for several month, could one expect the increase in
> the number of apoptotic cells due to spontaneous DNA
> Any comments and responses will be greatly
> Natalya Karp, M.D.
> McGill University, Dept. of Human Genetics
> Montreal, Quebec, Canada
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