Dr Montgomery,

I am using Clearite 3 and Propar for processing tissue in my lab at Leica.
The processing has been very good. I do have to change the xylene
substitute more often. I also up the number of xylene/substitute flushes in
the cleaning cycle. I would much prefer xylene for cleaning, but I cannot
use xylene in this building.

The staining of the tissue is very good also.

The only problem I have found with a substitute is it just doesn't work
like xylene. I guess that is why it is called a substitute!

Mari Ann Mailhiot BA HT
Applications Specialist
Leica Microsystems
847 317 7267




"Dr. Ian Montgomery"  on 04/26/2002 09:30:44
AM

To:   histonet@pathology.swmed.edu
cc:

Subject:  Xylene substitutes.


        The forces of darkness are moving on me and insist I move away from
xylene. So, from the many substitutes on the market what is the current
favourite? Although I'd like to hear all comments those from the UK would
be very welcome, just for ease of supply. At the moment I use Tissue-Clear
(Sakura) and mount from xylene using Entellan. Are any of these substitutes
miscible with Entellan, DPX and Balsam etc. or is it the 'own brand
mountant'.
Ian.
        .

Dr. Ian Montgomery,
Microscopy Service Unit,
Graham Kerr Building,
Institute of Biomedical & Life Sciences,
University of Glasgow,
Glasgow,
G12 8QQ.
Tel: 0141 332 8855
Office: 4652
Lab: 6644.
e-mail: ian.montgomery@bio.gla.ac.uk