Re: Xylene substitutes.


We have used Shandon's Xylene Substitute (Now called Histosolve) for the last 10 years.  We use it on our VIP and in the staining lines.  We use Shandon's mouting media also.  We love it and have had no problems.  We still keep some regular on hand to soak off coverslips and other things---but only order about 1 gallon a year.
Hope this helps.
Dawn Schneider, HT(ASCP)
Howard Young Medical Center
Woodruff, WI
-----Original Message-----
From: Dr. Ian Montgomery <>
To: <>
Date: Friday, April 26, 2002 9:39 AM
Subject: Xylene substitutes.

        The forces of darkness are moving on me and insist I move away from xylene. So, from the many substitutes on the market what is the current favourite? Although I'd like to hear all comments those from the UK would be very welcome, just for ease of supply. At the moment I use Tissue-Clear (Sakura) and mount from xylene using Entellan. Are any of these substitutes miscible with Entellan, DPX and Balsam etc. or is it the 'own brand mountant'.

Dr. Ian Montgomery,
Microscopy Service Unit,
Graham Kerr Building,
Institute of Biomedical & Life Sciences,
University of Glasgow,
G12 8QQ.
Tel: 0141 332 8855
Office: 4652
Lab: 6644.

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