amy,
did your researcher fix the samples before decal?
if they were not fixed you could have washed out the cells with all that
washing.
patsy

Amy Porter wrote:

> We are working on a research project involving mouse bone.  Trying to
> perform Luna's stain for eosinophils.  Tissues have been decaled with
> formic for a predetermined length of time by the researcher, then
> rinsed in running water for 4 hours and processed by standard
> methods.  The stain seems to be diffusely staining no matter the
> variations that we try, without much luck seeing the eosinophils.  All
> samples that are formalin fixed without decal are staining
> beautifully.  Any suggestions would be helpful.
>
> Amy S.Porter, HT(ASCP)
> Michigan State University
> College of Human Medicine
> portera203@yahoo.com
>
>
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