Re: Need help for decal samples


did your researcher fix the samples before decal?
if they were not fixed you could have washed out the cells with all that

Amy Porter wrote:

> We are working on a research project involving mouse bone.  Trying to
> perform Luna's stain for eosinophils.  Tissues have been decaled with
> formic for a predetermined length of time by the researcher, then
> rinsed in running water for 4 hours and processed by standard
> methods.  The stain seems to be diffusely staining no matter the
> variations that we try, without much luck seeing the eosinophils.  All
> samples that are formalin fixed without decal are staining
> beautifully.  Any suggestions would be helpful.
> Amy S.Porter, HT(ASCP)
> Michigan State University
> College of Human Medicine
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