> Are you using Chromic acid or Periodic acid?
> ----- Original Message -----
> From: "Weems, Joyce" 
> To: "'Johnston, Kathy'" ; "Histonet (E-mail)"
> 
> Sent: Friday, April 26, 2002 12:57 PM
> Subject: RE: Grocott problems
>
>
> > 1st of all - is your glass ware acid cleaned? 2nd - Do you have a
neighbor
> > that you could ask to do parallel stains - just to see if it might be
> > something in the processing? If the same thing happens at your
neighbor's,
> > then you'll know it's not just the stain.  Just my first thoughts.
> > Joyce Weems
> > Pathology Manager
> > Saint Joseph's Hospital of Atlanta
> >
> >
> > -----Original Message-----
> > From: Johnston, Kathy [SMTP:Kathy.Johnston@CLS.ab.ca]
> > Sent: Friday, April 26, 2002 1:34 PM
> > To: Histonet (E-mail)
> > Subject: Grocott problems
> >
> > We have been experiencing problems with our GMS stain over the past
> > few
> > weeks.  We run both a control slide containing fungal hyphae, and p.
> > carnii
> > to cover our bases.  Our problem is: it is taking much longer to
> > stain in
> > silver, and after we tone, and Na thio the slides, they are so pale
> > (or non
> > existent), that they are unacceptable.
> >
> > We have made new: 5% silver, 3% methenamine, 5% borax, stock silver
> > methenamine, 5% chromic acid, 0.1% gold chloride, and 2% sodium
> > thio.  All
> > to little avail other than to be able to make them acceptable to
> > sent out,
> > but not up to our usual standard.
> >
> > Any help on this very frustrating dilemma would be greatly
> > appreciated!
> >
> > Thanks in advance!
> >
> > Kathy Johnston
> > Tech II - Special Stains
> > Anatomic Pathology - Foothills Medical Center
> > Calgary Laboratory Services
> > Ph - 403-670-4760
> > Fax - 403-270-4093
> > kathy.johnston@cls.ab.ca
> >
> >
>