Re: GI biopsy blues
Rande,
I have used Mollifex for years and found it excellent for hard
tissues. One has to be careful not to leave the tissues in it for too long, as
it does work quite quickly, as the tissue swells. In experienced hands, it
is a very good product.
I have also used a diluted solution of fabric conditioner for
the same purpose. Does not have the alcohol content plus smells
nice!!
Evelyn Kaplan
Sultan Qaboos University,
Oman
----- Original Message -----
Sent: Friday, April 26, 2002 12:19
AM
Subject: Re: GI biopsy blues
Barbara,
One of the surfactants in the articles you are
describing is Mollifex, which is
now available in the U.S. If there
are any histo people from the UK or Canada
on the histonet, they may be
able to tell you more about it's use. The item
number for Mollifex is
65039-71 and can be ordered from a number of EM Science
distributors.
If you are interested, call me and I will give you the name
of
one.
Rande Kline
Technical Services
800-222-0342, ext.
443
Barbara.Davies@memhospcs.org on
04/25/2002 03:27:12 PM
To:
SEENRD@aol.com
cc: histonet@pathology.swmed.edu (bcc: Rande
Kline/EMI/Merck)
Subject: Re: GI biopsy
blues
Donna,
We have chronic problems with GI biopsies,
especially adenomatous polyps.
We first shortened the processing and
frankly, it did not help a great
deal. We have changed to
ProPar and pro Soft (replacing alcohols and
xylene on the processor) and
that has helped, but the biggest difference
was found when I discovered an
article from 1994 or 1996 Journal of
Histotechnology. A
surfactant was used to soak the blocks. We soak all
biopsy
blocks in a surfactant and it hasn't eliminated, but it has
substantially
reduced microchatter. (See the archives for our procedure
and the
correct reference from the Journal...I don't have it in front of
me right
now.)
Good luck to you,
Barb Davies
Memorial
Hospital
Colorado Springs, CO
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