Re: GI biopsy blues

From:"Ms. Evelyn Kaplan"

I have used Mollifex for years and found it excellent for hard tissues. One has to be careful not to leave the tissues in it for too long, as it does work quite quickly, as the tissue swells. In experienced hands, it is a very good product.
I have also used a diluted solution of fabric conditioner for the same purpose. Does not have the alcohol content plus smells nice!!
Evelyn Kaplan
Sultan Qaboos University,
----- Original Message -----
Cc: ;
Sent: Friday, April 26, 2002 12:19 AM
Subject: Re: GI biopsy blues

One of the surfactants in the articles you are describing is Mollifex, which is
now available in the U.S.  If there are any histo people from the UK or Canada
on the histonet, they may be able to tell you more about it's use.  The item
number for Mollifex is 65039-71 and can be ordered from a number of EM Science
distributors.  If you are interested, call me and I will give you the name of

Rande Kline
Technical Services
800-222-0342, ext. 443 on 04/25/2002 03:27:12 PM

cc: (bcc: Rande Kline/EMI/Merck)
Subject:  Re: GI biopsy blues


We have chronic problems with GI biopsies, especially adenomatous polyps.
We first shortened the processing and frankly, it did not help a great
deal.    We have changed to ProPar and pro Soft (replacing alcohols and
xylene on the processor) and that has helped, but the biggest difference
was found when I discovered an article from 1994 or 1996 Journal of
Histotechnology.   A surfactant was used to soak the blocks.   We soak all
biopsy blocks in a surfactant and it hasn't eliminated, but it has
substantially reduced microchatter.  (See the archives for our procedure
and the correct reference from the Journal...I don't have it in front of
me right now.)

Good luck to you,

Barb Davies
Memorial Hospital
Colorado Springs, CO

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