Diane,
there are specific antibodies to the collagen types, i prefer getting my
collagen antibodies from the U of Iowa Hybridoma Bank.  what animal species are
you working on?
Patsy

Boneslides@aol.com wrote:

> Can anyone recommend a stain that will differentiate different types of
> fibro-cartilage?  I am trying to differentiate the nucleus pulposis from
> annulus fibrosis in spine sections.  My understanding (which is VERY
> limited!!!) is that one is made of Type I collagen and the other is Type II
> collagen.
>
> Any help will be greatly appreciated!
>
> Thanks!
> Diane Mahovlic
> Orthopedic Pathology and Biomaterials Laboratory
> The Cleveland Clinic Doundation
> Cleveland, Ohio